Fig. 8
From: Low level laser therapy promotes bone regeneration by coupling angiogenesis and osteogenesis
LLLT promoting coupling angiogenesis and osteogenesis through ROS/HIF-1α pathway in the co-culture system. A The HIF-1α inhibitor DMBPA (50 μM) was added into the co-culture system. After 7 days of LLLT treatment, the angiogenesis related factors (VEGF and TGF-β) were detected by RT-qPCR. B After 21 days of LLLT treatment, the expression of angiogenesis-related factors (VEGF and TGF-β) was detected by RT-qPCR; C Detecting the mRNA expression of osteogenic-related factors (ALP and RUNX-2) by RT-qPCR at 7 days. D Detecting the osteogenesis-related factors (ALP and RUNX-2) by RT-qPCR at 21 days. E, G Detecting and analyzing the expression of osteogenesis-related protein (ALP and RUNX-2) by western blot at 7 days. F, H Detecting and analyzing the expression of osteogenesis-related protein (ALP and RUNX-2) by western blot at 21 days (ns, not statistically significant, *P< 0.05, **P< 0.01, ***P < 0.001)