Fig. 1

MiR-137 regulates NOTCH1-HES1 pathway by directly targeting NOTCH1. a Relative expression analyses of NOTCH1 and HES1 by qRT-PCR in transfected hASCs on 3 days, 7 days, and 14 days. b, c Western blotting (b) and band intensity analyses (c) of NOTCH1 and HES1 in transfected hASCs on 7 days. d The structure diagram of a luciferase reporter vector containing the 3′ UTR of NOTCH1-WT or NOTCH1-MT. e Softwares (TargetScan and RNA22) predicted the binding sites of miR-137 located in the 3′ UTR of NOTCH1-WT (underlined parts indicated the mutated bases in NOTCH1-MT). f Luciferase activity analyses of the NOTCH1-WT and NOTCH1-MT. Data are shown as mean ± SD of three independent experiments performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001 versus respective NC group