Fig. 3

NOTCH1 knockdown impairs osteogenesis by inducing HES1. a, b ALP staining (a) and quantification (b) of transfected hASCs after a 7-day culture in PM or OM (scale bar = 100 μm). c, d ARS staining (c) and quantification (d) of transfected hASCs after a 14-day culture in PM or OM. e Relative expression analyses of NOTCH1, HES1, and RUNX2 by qRT-PCR in transfected hASCs on 3 days, 7 days, and 14 days. f, g Western blotting (f) and band intensity analyses (g) of NOTCH1, HES1, and RUNX2 in transfected hASCs on 7 days. Data are shown as mean ± SD of three independent experiments performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001 versus respective NC group