Fig. 4

EGR1-dependent young-specific gene network module in human HSC: A Gene connections identified in young HSC compartment during EGR1-dependent WGCNA analysis (node and color nodes are proportional of node centrality, color, and size of the edge are proportional of edge centrality); B Network analysis in young HSC module with a number of direct edges on the first axis and logarithm base 10 of betweenness centrality and neighborhood connectivity for the size of the gene points; C misclassification error graph obtained in age supervised machine learning with genes of young HSC module; D barplot of predictive scores for predictive markers of young HSC; E single-cell expression of PDE4B, MYADM, and STK17B in human HSC stratified on age of donors (Fisher’s exact test was employed between the amount of positive cells in each group, numbers of positive cells, and mean expression of each marker are written in the corresponding color of groups)