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Table 1 Primers employed for gene expression analysis

From: MSCs from polytrauma patients: preliminary comparative study with MSCs from elective-surgery patients

Function

Gene

 

PRIMER ID

Housekeeping gene

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

Hs99999905

Adipogenic differentiation

CEBPα

CCAAT enhancer binding protein alpha

Hs00269972

Adipogenic differentiation

PPARγ

Peroxisome proliferator-activated receptor gamma

Hs01115512

Chondrogenic differentiation

SOX9

SRY-Box 9

Hs00165814

Chondrogenic differentiation

COL1A1

Collagen type I alpha 1 chain

Hs00164004

Osteogenic differentiation

ALPl

Alkaline phosphatase

Hs00758162

Osteogenic differentiation

RUNX2

Runt-related transcription factor

Hs00231692

Osteogenic differentiation

DKK1

Dickkopf WNT signaling pathway inhibitor 1

Hs00183740

Osteogenic differentiation

SPARC

Osteonectin

Hs00277762

Osteogenic differentiation

SPP1

Osteopontin

Hs00959010

Immunomodulation

TGF-β

Transforming growth factor beta 1

Hs00998133

Immunomodulation

TNFα

Tumor necrosis factor alfa

Hs00174128

Immunomodulation

PTGS2

Prostaglandin-endoperoxide synthase 2

Hs00153133

Immunomodulation

IL-6

Interleukin-6

Hs99999032

Immunomodulation

IL-10

Interleukin-10

Hs00174086

Immunomodulation

IDO

Indoleamine 2,3-dioxygenase 1

Hs00984148

Other

BDH2

3-Hydroxybutyrate dehydrogenase 2

Hs00560373