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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Mesenchymal stem cell-derived extracellular vesicles as probable triggers of radiation-induced heart disease

Fig. 1

Characterization of hp-MSCs and hp-MSC EVs. A Human placental tissue-derived mesenchymal stem cells (hp-MSCs) displayed identical fibroblast morphology. Representative images are shown. Scale bar: 200 μm. Representative histograms (B) and quantitative data (C) of flow cytometry analysis of the expressions of CD44, CD105, CD90, and CD73, but not CD45 and CD34 in hp-MSCs from two passages. D Representative images from electron microscopy show EVs (white arrow) from non-irradiated and irradiated hp-MSCs (n=1). Scale bar: 100 nm. E Nanoparticle track analysis of the size-distribution of EVs from non-irradiated and irradiated hp-MSCs (n=1). F Western blotting analysis of CD63 and TSG101 expression in EVs from non-irradiated and irradiated hp-MSCs (n=1). G Protein concentration of EVs from non-irradiated and irradiated hp-MSCs determined by Micro BCA protein assay. H EVs from three donors of hp-MSCs were pooled for miRNA sequences. Scatter plot image indicated miRNAs that were upregulated or downregulated more than two-fold in EVs from non-irradiated and irradiated hp-MSCs. The axis values indicate the expression values after normalization of miRNAs. Values are the mean ± SD. Non-irradiated-EVs: EVs isolated from conditioned medium of non-irradiated hp-MSCs, Irradiated-EVs: EVs isolated from conditioned medium of irradiated hp-MSCs

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