Fig. 5

IL-1β priming stimulate immunomodulatory property of MSC. A Concentration of IL-6, CCL2, and PGE2 (n = 8 and 8 in MSCn and MSCp groups respectively). Data are expressed with interquartile range. Mann-Whitney statistical analysis was used, **p < 0.01. B TNF-α, IL-6, and IL-1RA secretion levels from the LPS-activated THP-1 coculture with or without rat MSC naive (MSCn) or primed by 5 ng/mL of IL-1β (MSCp) from a pool of 8 donors or individual donors (MSCn or p 8 donors) at the ratio 1: 1 (1 MSC for 1 THP-1) up to a ratio of 1:50 were evaluated. The data are expressed as the median ± interquartile (n = 8 donors or 3 MSC replicates from the pool). Friedman’s tests for matched data (donor MSCn vs MSCp 8 donors) or Kruskal-Wallis for unpaired data (THP-1 vs MSC, and donors of MSC vs pool of MSCn, and MSCn vs MSCp) with Dunn’s correction were applied to assess the significance of observed differences *p < 0.05, **p < 0.01 or ^##p < 0.01, ^###p < 0.005