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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Mkx mediates tenogenic differentiation but incompletely inhibits the proliferation of hypoxic MSCs

Fig. 2

The Mkx content of AMSCs and BMSCs and the mRNA and protein expressions of Mkx and tenogenic differentiation markers after downregulation of Mkx by siRNA in BMSCs. qRT-PCR analysis (a) and representative western blots (b) of Mkx content in AMSCs and BMSCs. c qRT-PCR analysis of gene expression of Mkx after siRNA application in normoxia condition. d qRT-PCR analysis of gene expression of Mkx, Col-1a1, Col-3a1, Dcn, and Tnmd in the scramble group and the siRNA group in hypoxia condition. e Representative western blots of Mkx, Col-1a1, Col-3a1, Dcn, and Tnmd in the scramble group and the siRNA group in normoxia condition and in hypoxia condition. Representative immunofluorescence staining (f) of Mkx, Col-1a1, Col-3a1, Tnmd, and quantification data (g) in the scramble group and the siRNA group in hypoxia condition. Scale bar = 50 μm; magnification, × 400. Data were shown as mean ± SD. p < 0.05; p < 0.01

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