Fig. 2
From: Mkx mediates tenogenic differentiation but incompletely inhibits the proliferation of hypoxic MSCs
The Mkx content of AMSCs and BMSCs and the mRNA and protein expressions of Mkx and tenogenic differentiation markers after downregulation of Mkx by siRNA in BMSCs. qRT-PCR analysis (a) and representative western blots (b) of Mkx content in AMSCs and BMSCs. c qRT-PCR analysis of gene expression of Mkx after siRNA application in normoxia condition. d qRT-PCR analysis of gene expression of Mkx, Col-1a1, Col-3a1, Dcn, and Tnmd in the scramble group and the siRNA group in hypoxia condition. e Representative western blots of Mkx, Col-1a1, Col-3a1, Dcn, and Tnmd in the scramble group and the siRNA group in normoxia condition and in hypoxia condition. Representative immunofluorescence staining (f) of Mkx, Col-1a1, Col-3a1, Tnmd, and quantification data (g) in the scramble group and the siRNA group in hypoxia condition. Scale bar = 50 μm; magnification, × 400. Data were shown as mean ± SD. ★p < 0.05; ★★p < 0.01