Fig. 3

Histological changes in skeletal muscle tissue of Col6a1KO mice by COL6 supplementation. a Quantification of myofiber CSAs in the TA muscles of Col6a1KO mice 2 weeks (tx2w) and 12 weeks (tx12w) after medium injection or cell transplantation. Control WT mice are the same age as Col6a1KO mice. The x-axis shows the short axis of the muscle fiber CSA in μm. b The short axis of the myofiber CSAs in a shown as band graphs (blue, 56 μm ≤ CSA short axis; yellow, 26 μm ≤ CSA short axis ≤ 55 μm; red, CSA short axis ≤ 25 μm). *P < 0.05 vs WT, ^#P < 0.05 vs Col6a1KO, ^§P < 0.05 vs pMSC, ^†P < 0.05 vs iMSC. c CSA per single myofiber in TA muscles. Data are shown as the mean ± SD. For tx2w, n = 15 (WT), n = 10 (medium; Col6a1KO), n = 9 (pMSCs), n = 12 (iMSCs), and n = 7 (KO-iMSCs); for tw12w, n = 17 (WT), n = 11 (medium; Col6a1KO), n = 6 (pMSCs, iMSCs, and KO-iMSCs each). *P < 0.05. **P < 0.01