Fig. 1

Morphology, migration and distribution of hOPCs. A Schematic illustration of the experimental manipulation. P3 rat pups were used to prepare the hypoxic-ischemic model; hOPCs were injected into the transplant site and ZJU-37 was intraperitoneally injected at P12; rats were sacrificed immediately after the Morris water maze (MWM) test. B The chemical structure of ZJU-37. C Identification of the bright field morphology and immunofluorescence staining to detect the NPC or OPC marker A2B5 (green), the pre-oligodendrocyte marker NG2 (green), the OPC marker PDGFRα (green), the immature oligodendrocyte marker O4 (green) and the astrocyte marker GFAP (green) of transplanted hOPCs. Scale bar = 100 μm. D-E Transplanted cells stained with human-specific marker STEM121 (green) at 1 week and 12 weeks post-transplant in the hOPCs and ZJU-37 + hOPCs groups. F At higher magnification of the boxed area from the STEM121 + cells exhibited typical bipolar OPC morphology. Scale bar = 100 μm. n = 4 for each group