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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Engineered basic fibroblast growth factor-overexpressing human umbilical cord-derived mesenchymal stem cells improve the proliferation and neuronal differentiation of endogenous neural stem cells and functional recovery of spinal cord injury by activating the PI3K-Akt-GSK-3β signaling pathway

Fig. 2

Quality evaluation of bFGF-HUCMSCs. a Morphology of HUCMSCs and bFGF-HUCMSCs under a light microscope. Scale bars = 100 μm. b, c Surface marker expression of MSCs and bFGF-MSCs. They both expressed CD73, CD90, and CD105, but did not express CD11b, CD19, CD34, CD45, or HLA-DR in FCM analysis. No significant differences were observed between MSCs and bFGF-HUCMSCs (p > 0.05). d Adipogenic, osteogenic, and chondrogenic differentiation was assayed by Oil Red O (Scale bars = 50 μm), Alizarin Red (Scale bars = 100 μm), and Alcian Blue (Scale bars = 200 μm) staining, respectively. e, g Quantified data of the Oil Red O staining area and Alizarin Red staining of MSCs and bFGF-MSCs by ImageJ software. f Karyotype analysis showed a normal karyotype (X, X) in bFGF-HUCMSCs without any karyotype variation

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Stem Cell Research & Therapy

ISSN: 1757-6512