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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Engineered basic fibroblast growth factor-overexpressing human umbilical cord-derived mesenchymal stem cells improve the proliferation and neuronal differentiation of endogenous neural stem cells and functional recovery of spinal cord injury by activating the PI3K-Akt-GSK-3β signaling pathway

Fig. 8

a Western blot analysis of the phosphorylation level of PI3K, AKT, and GSK3β in mice with an injured spinal cord. b Western blot analysis of the phosphorylation levels of AKT, GSK3β, and ERK1/2 in NSCs treated with HUCMSCs or bFGF-HUCMSCs culture supernatant at the indicated time points (0, 15, 30, 60, 180, and 360 min). c Phosphorylation levels of AKT, and GSK3β in LY294002-pretreated NSCs treated with conditioned medium of HUCMSCs or bFGF-HUCMSCs for 60 min. d EDU staining of LY294002-pretreated NSCs cocultured with HUMSCs or bFGF-HUCMSCs. Scale bars = 50 μm. e Immunofluorescence staining of Tuj-1 (green) and GFAP (red) in LY294002-pretreated NSCs cocultured with HUCMSCs or bFGF-HUCMSCs. Scale bars = 50 μm. f, g EDU staining and Tuj/GFAP staining of LY294002-pretreated NSCs cocultured with bFGF-HUCMSCs or HUCMSCs. **p < 0.01 and ***p < 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512