Fig. 1
From: Antifibrotic effect of lung-resident progenitor cells with high aldehyde dehydrogenase activity
Detection of cell population with high ALDH activity in the mouse lung. Lung cells obtained from 6-to-8-week-old wild-type C57BL/6 mice were analyzed using flow cytometry. Data were analyzed using the FACS Diva (BD Biosciences) and FlowJo (version 10.7.1, BD Biosciences) software. A ALDHbr gating determined by comparing samples stained with ALDEFLUOR alone and with ALDEFLUOR and diethylaminobenzaldehyde (DEAB), a specific inhibitor of ALDH. B–D ALDHbr cells observed in the whole lung, in the nonhematopoietic/lung-resident CD45− fraction, and in the hematopoietic CD45+ fraction. E CD45−/ALDHbr cells divided further into EpCAM+ and PDGFRα+ fractions