Fig. 1

FoxC1 is highly expressed in the ischemic ECs. A Heatmap of gene expression levels of 84 TFs. The top 10 highly expressed TFs are listed. Downregulated genes are represented in blue, and upregulated genes are represented in red. B, C The mRNA and protein expression levels of these TFs were verified in both cultured ECs using qRT-PCR (B) and immunoblotting (C). All data are the means ± SEM. p < 0.05: *versus Normoxia (n = 10 per group), One-way ANOVA analysis was used for statistical analysis. D, E Infarcted heart tissues were stained for immunohistochemistry. Representative images are shown in the infarct, peri-infarct, and remote areas (D), and the statistical ratios of FoxC1 highly expressing cells are shown in E. FoxC1 was positively stained as brown in the infarcted hearts, and highly seen in the cytoplasm of blood vascular cells around the peri-infarct areas. Scale bars: 50 µm. All Data are shown as means ± SEM. **p < 0.05 (n = 10 per group), Welch ANOVA analyses were performed for statistical analysis