Fig. 3

Feline ASCs downregulate CD8+ T cell surface expression, increase CD25 and CD57 expression, and shift CD8+ T cells toward a terminally differentiated, effector phenotype. Representative flow-plot on CD8+ T cell surface expression on A non-activated CD8+ T cells B activated CD8+ T cells C activated CD8+ T cells co-cultured with MSCs. D Summary of percentage of CD8lo T cells (n = 5, p-value = 0.007). CD57 expression on E activated CD8+ T cells F activated CD8+ T cells co-cultured with MSCs G Summary of percentage of ASCs’ induction of CD57+ CD8lo T cells (n = 5, p value = 0.007). CD25 and CD62L expression on H non-activated CD8+ T cells I activated CD8+ T cells J activated CD8+ T cells co-cultured with MSCs K Summary of CD25 and CD62L expression. ASCs increase activation (CD25+) and shedding of L-selectin (CD62L−) on CD8+ T cells (n = 5, p-value = 0.028). Expression of CD45R and CD62L to distinguish between effector, effector memory, central memory and naïve CD8+ T cells in L non-activated CD8+ T cells M activated CD8+ T cells N activated CD8+ T cells co-cultured with MSCs. O Summary of increase effector phenotype (CD45R+ , CD62L-) on CD8+ T cells induced by ASCs (n = 5, p-value = 0.02)