Fig. 4

Feline ASCs do not induce senescence in CD8+ T cells. Histogram depicting mean fluorescence intensity for senescence-associated β-galactosidase (SA-β-Gal) positive CD8+ T cells in A PBMCs alone and PBMCs in co-culture with MSCs B in activated PBMCs and activated PBMCs in co-culture with MSCs C Summary of SA-β-Gal activity with no significant increase between activated PBMCs and activated co-cultures and indication of SA-β-Gal activity was mostly from mitogen activation (n = 7, p-value = 0.007). Fold change calculated based on PBMCs with ConA