Fig. 5

NF-κB signaling and glycolysis are shared pathways between in vivo and in vitro models. A KEGG pathway analysis of the overlapped DEGs between Con A + hWJ-MSCs versus Con A + PBS group, Con A + PBS versus control group, activated T cells co-cultured with hWJ-MSCs versus activated T cells and activated T cells versus naïve T cells. B and C Heatmap showing expression of key genes of NF-κB signaling pathway (B) and glycolysis (C) in indicated groups. D RT-qPCR for key genes involved in glycolysis in mouse liver tissues in indicated groups (n = 5/group). E and F Western blotting (E) and corresponding densitometry analysis (F) for key genes involved in glycolysis in mouse liver tissues in indicated groups (n = 4/group). Data in D and F is shown as mean ± SD with the indicated significance (*p < 0.05, **p < 0.01, ***p < 0.001). KEGG, Kyoto Encyclopaedia of Genes and Genomes