Fig. 8
Mouse model showed P-ADSC improved perfusion recovery, attenuated degeneration and strengthened angiogenesis of ischemic hindlimb. A Representative laser perfusion Doppler image (LDPI) of mouse hindlimb before and after surgery. B Blood perfusion recovery in limb ischemia of each group at days 3, 7, 14, 21, post-surgery. C Quantitative analysis of blood recovery measured by LDPI. Blood recovery was represented using the ratio of ischemic hindlimb perfusion to contralateral hindlimb perfusion. D Haematoxylin–eosin (H&E) staining of gastrocnemius muscle of mouse ischemic hindlimb. E, F immunofluorescent staining of CD31 + capillaries in gastrocnemius muscles of ischemic tissue and quantification analysis. *p < 0.05, **p < 0.01 versus Saline, #p < 0.05, ##p < 0.01 versus ADSC. P-ADSC PEV-treated ADSCs