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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: The effect of genetically modified platelet-derived growth factor-BB over-expressing mesenchymal stromal cells during core decompression for steroid-associated osteonecrosis of the femoral head in rabbits

Fig. 1

Methods for histology field selection. Histology slides of the femoral head were imaged using a Keyence light microscope at ×40 magnification to visualize the entirety of each specimen. a Representative image of an H&E stained specimen with the borders of the CD area outlined with a dashed yellow quadrilateral. b The same image is used to demonstrate the position of the four fields that were used to image the subchondral bone at ×200 magnification for H&E, TRAP, and CD31 stained sections. c The same image is used to demonstrate the position of the three fields that were used to image the bone surrounding the bone tunnel at ×200 magnification for H&E, TRAP, and CD31 stained sections. d The same image is used to demonstrate the position of the three fields that were used to image the newly formed bone within the bone tunnel at ×200 magnification for the CD31 stained sections. Scale bar: 1000 µm. H&E hematoxylin and eosin, CD, core decompression, TRAP tartrate-resistant acid phosphatase, CD31 cluster of differentiation 31

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Stem Cell Research & Therapy

ISSN: 1757-6512