Fig. 2

Characterization of induced pluripotent stem cells corrected by genomic editing. a DNA sequencing at position 6055 of exon 41 in LRRK2. HDR generated two silent mutations introduced by donor DNA. b Bright-field image and immunofluorescent staining for OCT4 (green) and TRA-1-81 (red) in corrected iPSCs (ABE01). Nuclei are shown by DAPI staining (blue). Scale bar = 100 µm. c Karyotype of ABE01. d Teratoma formed after subcutaneous injection of ABE01 into NUD mice. e DA neurons derived from ABE01 co-expressing TUBB3 (green) and TH (red). Nuclei are shown by DAPI staining (blue). Scale bar = 100 µm