Fig. 6

Flow cytometric analysis of B10, Th1, Th2, Th17 and Treg cells in the spleens of MRL/lpr mice treated by MSCs with TGF-β1 low expression. a, c, e, g, i Representative FACS images of CD19⁺IL-10⁺ (B10 cells), CD4⁺IFN-γ⁺ (Th1 cells), CD4⁺IL4⁺ (Th2 cells), CD4⁺IL17⁺ (Th17 cells) and CD4⁺Foxp3⁺ (Treg cells). For B10 cells detection, B cells were isolated from the spleens of mice and were stimulated for 48 h with sCD40 Ligand and CpG. b, d, f, h, j Percentages of B10, Th1, Th2, Th17 and Treg cells in the spleen of MRL/lpr mice treated by MSCs with TGF-β1 low expression. Data are mean ± SD. MSCs-shRNA-Con (n = 7), MSCs-shRNA-TGF-β1 (n = 7). *p < 0.05, **p < 0.01. MSCs-shRNA-Con, MSCs infected by control lentivirus; MSCs-shRNA-TGF-β1, MSCs infected by lentivirus with TGF-β1 shRNA