Fig. 5

A Immunogenicity of ASCs derived from MZ twin donors using one-way MLR analysis. Peripheral blood mononuclear cell (PBMC) proliferation after 5 days of coculturing ASCs with PBMCs. Phytohemagglutinin-M (PHA). B Immunosuppressive capacity of ASCs derived from MZ twin donors in direct cocultures with PBMCs. PBMC proliferation was suppressed in direct cultures but not in C indirect cultures. D Relative gene expression of IL-6 and E TNF. Statistical analyses were performed using multilevel mixed-effect regression model test for the nested data structure for immunosuppression in direct cocultures and relative gene expression of IL-6 and TNF, and Wilcoxon signed-rank test for immunogenicity and immunosuppression in indirect cocultures. Significance: * = p < 0.05. All quantitative data are presented as the mean ± SD