Fig. 3

Effects of chemotherapy and hASC transplantation on body weight and ovarian morphological features. A, B Body weight of CTR, CH and IV females, following both light (L) and heavy (H) chemotherapeutic treatments at the indicated time points; sample size: n = 3 and n ≥ 16, respectively. Values are expressed as mean ± SD. C, D top panel Ovarian weight at week 4. Values are displayed as scattered dot plot showing mean ± SD. Statistical significance is calculated with One-Way ANOVA vs CTR (letters) and vs CH (asterisks) as described in “Methods” section. Sample size: n = 3 and n ≥ 8 for light (L) and heavy (H) chemotherapeutic treatment, respectively. C, D bottom panel Representative micrographs of ovaries at week 4. Distance between ruler ticks = 1 mm. E, F Representative midsagittal H&E stained sections of w4 ovaries showing the hilum region. Scale bar = 500 μm. L-IV and H-IV images are combined because histological sections exceeded camera field of view. G, H Representative IF images of the hilum region following αSMA⁺ staining. Scale bar = 50 μm. I, J Quantification of the MVD as the percentage of αSMA⁺ area compared to the area of the whole section. Values are displayed as scattered dot plot showing mean ± SD. Statistical significance is calculated with t-test (w0) and with One-way ANOVA (w4) vs CTR (letters) and vs CH (asterisks) as described in "Methods". Sample size: n = 3 and n ≥ 3 for light (L) and heavy (H) chemotherapeutic treatment, respectively