Fig. 3From: Novel culture media enhances mononuclear cells from patients with chronic limb-threatening ischemia to increase vasculogenesis and anti-inflammatory effectThe percentages of CD4+CD25+CD127+ (inactivated T regulatory cells) in peripheral blood mononuclear cells (PBMNCs) compared between PBMNCs cultured in Quality and Quantity culture media (QQ-media) and PBMNCs cultured in standard culture media. All experiments were performed in triplicate. Forward and side scatter plots were used to gate the lymphocyte populations. The gated cells were then evaluated to identify CD3+CD4+ cells. The CD3+CD4+ cells were then gated to assess the populations of CD4+CD25+and CD127+ cells. Student's t-test was used for phenotypic analysisBack to article page