Fig. 4

SPION-MSCs promote macrophage polarization towards the M2 phenotype under sepsis-induced liver injury in mice. A, B Flow cytometry plots and an analysis of the percentage of CD11b + F4/80 + macrophages and the M2 marker CD206. C Immunofluorescence of macrophages and their M2 CD206 markers in the whole liver. D Representative images of western blots to assess the levels of iNOS, IL-10 and Arg1 in the livers of mice. Data with error bars are presented as the mean ± SD. Each panel is a representative experiment of at least three independent biological replicates. Scale bars, 50 mm. *p < 0.05, **p < 0.01, ***p < 0.001 as determined by unpaired Student’s t-test