Fig. 4

Chromosomal distribution of VIS and its bias for transcriptionally active genes. a Circos plot shows genomic location of all 897 VIS from mice m599, m598, and m591. Box plots in the center show maximum frequency of mCherry-H5 (filled red dots) and EGFP-WT (filled green dots) VIS clones in mice m599, m598, and m591 over 6 weeks. Genomic location of mCherry-H5 (filled red dots) and EGFP-WT (filled green dots) VIS clones is plotted on three concentric circles depending on their maximum frequency over 6 weeks: Low-frequency clones with maximum frequency below the first quartile value (innermost concentric circle), High-frequency clones above third quartile value (outermost concentric circle), and medium-frequency clones between first and third quartile (middle concentric circle). Top 10 high-frequency VIS clones are shown in darker colors. Color-coded short line segments under each VIS show gene biotypes of VIS-proximal genes. Gene symbols above ideograms represent genes proximal to the top 10 VIS clones from mice m599 (blue), m598 (brown), and m591 (light pink). b Classification of all, persistent, and Top 10 VIS clones based on biotype of VIS-proximal gene. Inner pie chart shows clones classified based on gene biotype of the most proximal gene. Outer Donut plots show number of VIS (in bracket % of VIS) proximal to active (dark color) or inactive (faded colors) genes. Active proximal genes have FPKM > 1