Fig. 1
From: Noncanonical Wnt5a signaling regulates tendon stem/progenitor cells senescence
Increased Wnt5a expression correlates with a switch from canonical to noncanonical Wnt signaling. a Heatmap showed the changes in the expression of Wnt genes in young and aged TSPCs. b Relative mRNA levels of Wnt5a in TSPCs were investigated by qRT-PCR. c Western blotting for the Wnt5a protein levels in young and aged TSPCs. d Immunofluorescence staining for Wnt5a in young and aged TSPCs. Scale bar: 20 μm. e, f Immunofluorescence staining for β-catenin in young and aged TSPCs. Scale bar: 20 μm. g Western blotting for β-catenin protein levels in young TSPCs, aged TSPCs and young TSPCs treated with Wnt5a (100 ng/ml). h, i qRT-PCR for Axin2 and Lgr5 mRNA levels in young and aged TSPCs. j, k Axin2 and Lgr5 mRNA levels in young TSPCs and young TSPCs treated with Wnt5a. Values represent the mean ± SD. *p < 0.05, significantly different from the young group