Fig. 7

Fluorescent imaging of lipid peroxidation in MSCs exposed to high HCTs. a Unstained control, b negative control staining for normal lipids (no peroxidation, red), c positive lipid peroxidation control, MSCs treated for 2 h with cumene hydroperoxide (green signal). MSCs incubated for 48 h prior to staining with d 10% HCT, e 20% HCT and f 40% HCT show evidence of lipid peroxidation (green) and cell detachment (rounded signals) and dampening of red fluorescence. Unstained cells in d–f are residual RBCs post-washing. Scale bar = 200 microns. For all micrographs: total magnification = 520×