Fig. 3

PD-MSCs^PRL−1 regulated calcium influx in rat hepatocytes. a mRNA and b, c protein levels of SERCA2b in TG (500 nM)-treated WB-F344 cells cocultured with PD-MSCs or PD-MSCs^PRL−1 for 24 h. GAPDH was used as a loading control. d Representative fluorescence images and e–g the fluorescence intensities of calcium influx using ER-LAR GEGO for ER (red), Y-GECO1 for cytoplasm (yellow), and mt-GEM-GECO1 for mitochondria (cyan blue) in TG (500 nM)-treated WB-F344 cells cocultured with PD-MSCs or PD-MSCs^PRL−1 for 24 h. Data from each group are shown as the means ± SD and were assessed using Student’s t test. *p < 0.05 vs. NTx, #p< 0.05 vs. PD-MSCs. GAPDH, glycraldehyde-3-phosphate dehydrogenase; NTx, nontransplantation; PRL-1, phosphatase of regenerating liver-1; SERCA2b, sarco/endoplasmic reticulum Ca²⁺ ATPase; TG, thapsigargin