Fig. 1

Osteogenic differentiation of PDLSCs under osteogenic medium with different glucose concentrations. PDLSCs were cultured in osteogenic medium containing glucose at concentrations of 5.5 mmol/L, 11 mmol/L, 25 mmol/L, 44 mmol/L. a Osteogenic differentiation was determined by alizarin red staining and ALP staining after 21 days; Quantitative measurement of mineralization, and the absorbance was measured at 595 nm by spectrophotometry (****p < 0. 0001); b RUNX2 and SNAI2 was measured by Western Blot at 14 days of osteogenic induction. β-actin as an internal reference. And Quantitative analysis of expression levels (****p < 0. 0001); c the expression of RUNX2 and SNAI2 in PDLSCs were evaluated by RT-PCR at 7 days of osteogenic induction. The data are expressed as the mean ± SD of at least three replicates, *p < 0. 05; **p < 0. 01; ***p < 0. 001; ****p < 0. 0001, all results were obtained from at least three independent experiments. PDLSCs periodontal ligament stem cells, RT-PCR real-time quantitative polymerase chain reaction, SD standard deviation