Fig. 2

High glucose promoted sumoylation of IGF-1R. 5.5 mmol/L and 25 mmol/L groups were selected for follow-up experiment. a IGF-1R and SUMO1 was measured by Western Blot at 7 days of osteogenic induction. β-actin as an internal reference. And Quantitative analysis of expression levels (****p < 0. 0001). b Sumoylation of IGF-1R was detected by immunoprecipitation (IP) with anti-IGF-1R antibody followed by Western Blot with anti-SUMO1 antibody. IGF-1R was conjugated with SUMO1 in PDLSCs. c Sumoylation of IGF-1R were determined by IP of IGF-1R and IB for IGF-1R. The data are expressed as the mean ± SD of at least three replicates, *p < 0. 05; **p < 0. 01; ***p < 0. 001; ****p < 0. 0001, all results were obtained from at least three independent experiments