Fig. 4

Inhibition of IGF-1R in high glucose environment promoted osteogenic differentiation of PDLSCs. a The effects of NVP-ADW742 treatment on cell survival rate were assayed by CCK-8 assay. Cells were cultured for 24 h, 48 h, and 72 h in the concentrations of NVP-ADW742, from 0 to 50 umol/L. The results were obtained from at least three independent experiments. b IGF-1R, RUNX2 and SNAI2 were measured by Western Blot at 7 days of osteogenic induction. β-actin as an internal reference. c Osteogenic differentiation was determined by alizarin red staining and ALP staining after 21 days; Quantitative measurement of mineralization, and the absorbance was measured at 595 nm by spectrophotometry; d Western Blot was performed and found that NVP-ADW742 selectively inhibited the PI3K/AKT signing pathway, which related to the level of IGF-1R. All results were obtained from at least three independent experiments. PI3K, phosphatidylinositol-3-kinase; AKT, protein kinase B. The data are expressed as the mean ± SD of at least three replicates, *p < 0. 05; **p < 0. 01; ***p < 0. 001; ****p < 0. 0001, all results were obtained from at least three independent experiments