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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Valproic acid promotes the in vitro differentiation of human pluripotent stem cells into spermatogonial stem cell-like cells

Fig. 1

VPA promoted the differentiation of hiPSCs into SSCLCs. A Immunostaining of PLZF (green) of differentiated cells using SSCLC induction medium containing different concentrations of VPA and/or VC at 12 days of differentiation, and the nuclei were stained with DAPI (blue). B The percentage of PLZF+ cells, representing the SSCLC induction efficiency, was detected by flow cytometry at 12 days of differentiation. C The percentage of SSCLCs and the expression of PLZF (detected by RT-qPCR) in different groups, n = 3, *p < 0.05 when compared with 0.5 (VPA + VC)

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Stem Cell Research & Therapy

ISSN: 1757-6512