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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Valproic acid promotes the in vitro differentiation of human pluripotent stem cells into spermatogonial stem cell-like cells

Fig. 3

The high concentration of VPA induced further differentiation. A PI was used to stain DNA and the percentage of haploid cells at 12 days of differentiation was detected by flow cytometry. B immunostaining of Acrosin (red), TNP1 (red) and SYCP3 (red) of differentiated cells using developed SSCLC induction protocol at 12 days of differentiation, and the nuclei were stained with DAPI (blue). C the expression of meiosis-related genes detected by RT-qPCR at 12 days of differentiation, n = 3, *p < 0.05 when compared with VPA and # p < 0.05 when compared with VPA + VC

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Stem Cell Research & Therapy

ISSN: 1757-6512