Skip to main content

Table 4 Neutrophil activation in co-culture with MSCs

From: Immune response to allogeneic equine mesenchymal stromal cells

  6 h 12 h
1:1 1:10 1:100 Control 1:1 1:10 1:100 Control
Autologous 0.53
(0.09)a
0.50
(0.05)a
0.66
(0.17)a
10.3
(2.4)a,b
4.5
(0.4)a,b
5.1 (0.4)a,b
Blood donor 5.76
(3.17)b
2.40
(1.75)b
1.00
(0.34)a
5.1
(1.5)a
1.0
(0.6)a
3.2 (2.4)a
MHC II-high 3.04
(0.08)b,c
0.92
(0.30)a,b
1.42
(0.31)b
12.5
(12.9)b
6.4
(6.0)b
9.3 (2.3)b
MHC II-low 1.78
(0.82)a,c
0.99
(0.41)a,b
0.99
(0.37)a
6.4
(3.7)a,b
3.2
(1.7)a,b
5.4 (2.8)a,b
PBMC 0.21
(0.20)
9.3 (9.8)
Activated PBMC 99.9
(0.1)
99.5 (1.6)
  1. Median percent of neutrophils activated and the IQR is shown for co-cultures with the ratio of MSCs/neutrophils and for neutrophils cultured alone. Values within each column which have different letters are significantly different (p < 0.05). Autologous, autologous MSC co-culture (n = 1); blood donor, universal blood donor MSC co-culture (n = 3); MHC II high, MHC II-high MSC co-culture (n = 3); MHC II Low, MHC II-low MSC co-culture (n = 3); neutrophils, neutrophils alone; activated neutrophils, neutrophils activated with 2.5 µM phorbol myristate acetate (PMA)