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Table 4 Neutrophil activation in co-culture with MSCs

From: Immune response to allogeneic equine mesenchymal stromal cells

 

6 h

12 h

1:1

1:10

1:100

Control

1:1

1:10

1:100

Control

Autologous

0.53

(0.09)a

0.50

(0.05)a

0.66

(0.17)a

–

10.3

(2.4)a,b

4.5

(0.4)a,b

5.1 (0.4)a,b

–

Blood donor

5.76

(3.17)b

2.40

(1.75)b

1.00

(0.34)a

–

5.1

(1.5)a

1.0

(0.6)a

3.2 (2.4)a

–

MHC II-high

3.04

(0.08)b,c

0.92

(0.30)a,b

1.42

(0.31)b

–

12.5

(12.9)b

6.4

(6.0)b

9.3 (2.3)b

–

MHC II-low

1.78

(0.82)a,c

0.99

(0.41)a,b

0.99

(0.37)a

–

6.4

(3.7)a,b

3.2

(1.7)a,b

5.4 (2.8)a,b

–

PBMC

–

–

–

0.21

(0.20)

–

–

–

9.3 (9.8)

Activated PBMC

–

–

–

99.9

(0.1)

–

–

–

99.5 (1.6)

  1. Median percent of neutrophils activated and the IQR is shown for co-cultures with the ratio of MSCs/neutrophils and for neutrophils cultured alone. Values within each column which have different letters are significantly different (p < 0.05). Autologous, autologous MSC co-culture (n = 1); blood donor, universal blood donor MSC co-culture (n = 3); MHC II high, MHC II-high MSC co-culture (n = 3); MHC II Low, MHC II-low MSC co-culture (n = 3); neutrophils, neutrophils alone; activated neutrophils, neutrophils activated with 2.5 µM phorbol myristate acetate (PMA)