Fig. 5

SAP supplementation alleviated the apoptosis of WJMSCs encapsulated with PF-127. a The cell apoptosis was detected by TUNEL immunofluorescence in different groups. TUNEL, green, represents apoptotic cell; DAPI, blue, represents cell nuclei. Scale bar, 100 μm. b The quantitative data of the percentage of TUNEL-positive cell per field were analyzed. Error bars represent mean ± SEM; n = 3 independent experiments. Significance was determined using one-way ANOVA. *p < 0.05, **p < 0.01. c The mRNA level of proapoptotic and antiapoptotic gene was measured by qRT-PCR in different groups. Error bars represent mean ± SEM; n = 3 independent experiments. Significance was determined using one-way ANOVA. *p < 0.05, **p < 0.01. d The protein level of proapoptotic and antiapoptotic gene was measured by western blot in different groups. e The quantitative data of protein level of different genes in different groups. Error bars represent mean ± SEM; n = 3 independent experiments. Significance was determined using one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001. f CMH2DCFAD staining was analyzed in different groups, CMH2DCFAD: green, represents the ROS fluorescence intensity; Hoechst, blue, represents cell nuclei. Scale bar: 20 μm. g The quantitative data of the percentage of CMH2DCFAD-positive cell per field were analyzed. Error bars represent mean ± SEM; n = 3 independent experiments. Significance was determined using one-way ANOVA. ***p < 0.001. h Mitochondrial location and distribution patterns were detected in different groups. Scale bar, 20 μm. Enlarge scale bar, 10 μm