Fig. 1

PPARβ/δ agonist promotes osteogenic differentiation and decreases the apoptosis in high-glucose environment. A, B ALP staining and ALP activity quantitative analyses of rBMSCs after 7 days of osteogenic induction treated with GW501516 of different concentration (scale bar = 500 µm). C, D ARS staining and quantitative analyses of rBMSCs after 21 days of osteogenic induction treated with GW501516 of different concentration (scale bar = 500 µm). E qRT-PCR for the osteogenesis-related genes Runx2, Bglap, Col1a1 and Osx after 7 days of osteogenic induction. F Analysis of cells stained with Annexin V/PI by flow cytometry. Data are expressed as mean ± SD. The p values were calculated by two-way ANOVA with Tukey’s post hoc test. (NS, not statistically significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)