Fig. 5

Photoreceptor degeneration or FINS treatment induces ciliary epithelial cell migration into the neural retina. A-I Analyses of eye slides from the endpoint of the experimental paradigm outlined in Fig. 4A (20 sections analyzed per eye). A Percent of eye sections with Msx1-tdTomato⁺ cells present in retina for mice of the indicated conditions. There was a significant interaction between injury and treatment on migration frequency (p = 0.021; N = 6–8 eyes per group). Each data point represents a single eye. B, C Quantification of the migration distance of Msx1-tdTomato⁺ cells into the retina for mice of the indicated conditions. B The migration distance recorded for individual cells. Each data point represents a single cell, N = 9–228 cells from 6–8 eyes per group. c Average migration distance per eye. There was a significant effect of treatment (p = 0.029) and injury (p = 0.028). N = 6–8 eyes per group. Each data point represents a single eye. D-E Quantification of the number of Msx1-tdTomato⁺ cells in the retina for mice of the indicated conditions. D The total number of cells recorded in individual retina sections. Each data point represents a single retina section with at least one Msx1-tdTomato⁺ cell detected. e Average number of Msx1-tdTomato⁺ cells in retina sections per eye. There was a significant interaction between injury and treatment on the number of Msx1-tdTomato⁺ cells in the retina (p = 0.007; N = 6–8 eyes per group). Each data point represents a single eye. F-I Brightfield and fluorescence overlay images of Msx1-tdTomato⁺ cells in retinas from mice treated with the indicated conditions. White arrows indicate Msx1-tdTomato⁺ cells in the retina. J-M Msx1-tdTomato⁺ CE cells in the retina with neuroepithelial or neurite morphology at Day 31. DRAQ5 stain was used to label all nuclei. Straight dashed line indicates the border between the ciliary epithelium (CE) and the neural retina (NR). Dashed line box indicates high magnification inset. Statistics: Two-way ANOVAs with Holm-Sidak posthoc tests. Means ± SEMs indicated. * = p < 0.05