Fig. 7

mTORC2 is necessary for TM9SF4-mediated osteogenesis. A, B The protein level of pAkt was detected via Western blot after osteogenic induction. C Primary MSCs with siRNA transfection were cultured in osteogenic medium for 1w before RNA detection. Error bars represent mean ± SEM, n = 3. **P < 0.01, ***P < 0.001, ****P < 0.0001, ns = not significant. D–G After siRNA transfection, primary MSCs were induced with osteogenic medium for 2w and MC3T3-E1 cells for 10d before Alizarin Red S staining. Scale bars = 500 µm. Error bars represent mean ± SEM, n = 3. **P < 0.01, ***P < 0.001, ****P < 0.0001 (E, G). H After siRNA transfection, primary MSCs were cultured in osteogenic medium for 1w before Western blot detection