Fig. 2

A comparison of two methods for the biofunctionalisation of bacterial cellulose with a model protein shows improved results for the ‘one pot’ method. A Both conventional (left) and aqueous one-pot (right) strategies were used to prepare glycido-coated cellulose through silanisation followed by covalent adherence of protein. AlexaFluor®-488-conjugated antibody immobilised onto the cellulose modified by (B1) conventional method results in cracking of protein, as shown by arrows but not in (B2) using the ‘aqueous one pot’ method. Surface fluorescence intensity profiles show an increase in intensity in regions where AlexaFluor®-488-conjugated antibody has covalently bound to cellulose (C1, C2 representing conventional and aqueous one pot strategy, respectively). The fluorescence map was obtained by plotting the intensity profile of the conjugated antibody attached to BC