Fig. 2

Hematopoietic progenitors detection in Casepase-3 gene knockout mice. A Flow cytometry plots of bone marrow cells from wild-type (WT) and Caspase-3^−/− (KO) mice, showing the gates on LSK (Lin⁻Sca-1⁺c-kit⁺), LT-HSC (Lin⁻Sca-1⁺c-kit⁺CD34^lowFlk2^low), ST-HSC (Lin⁻Sca-1⁺c-kit⁺CD34⁺Flk2^low), MPP (Lin⁻Sca-1⁺c-kit⁺CD34⁺Flk2⁺), CMP (Lin⁻Sca-1⁻c-kit⁺CD34⁺CD16/32^low), GMP(Lin⁻Sca-1⁻c-kit⁺CD34⁺CD16/32⁺), and MEP(Lin⁻Sca-1⁻c-kit⁺CD34 ^low CD16/32^low). B Flow cytometry analysis of the percentage of LSK cells and the number of total cells, LSK, LT-HSC, ST-HSC, and MPP in the bone marrow of WT and Caspase-3 KO mice (n = 4). C Morphology of colony-forming units of granulocyte–macrophage colonies (CFU-G, CFU-M, and CFU-GM). D Analysis of total colony numbers (including CFU-E, BFU-E, CFU-G, CFU-M, CFU-GM, and CFU-GEMM) at 7 and 14 days for the detection of HSPCs isolated from WT and Caspase-3 KO mice (n = 3–5). E Analysis of myeloid colony numbers (including CFU-G, CFU-M, and CFU-GM) at 14 days for the detection of HSPCs isolated from WT and Caspase-3 KO mice (WT: n = 4; KO: n = 3). (F-G) Bone marrow cells from WT and Caspase-3 KO mice (CD45.2) were isolated and injected into lethally irradiated recipients (CD45.1). Engraftment efficiency in recipient mice was assessed according to the donor contribution of CD45.2⁺ cells by flow cytometry. F The analysis of peripheral blood cells was performed every four weeks post-transplantation (WT: n = 3; KO: n = 5). G The assessment of progenitor subpopulations in the bone marrow was performed at 16 weeks after mice sacrifice (WT: n = 3; KO: n = 5). All data are presented as mean ± SEM. ns: not significant, *P < 0.05, **P < 0.01, ****P < 0.0001