Fig. 4

SCF and CXCL12 level determination in BA-MSCs after Casepase-3 gene knockout. A Gene expression of SCF in wild-type (WT) and Caspase-3^−/− (KO) BA-MSCs by qRT-PCR (n = 6), and ELISA analysis in the supernatants after culture for 7 days (n = 4). B Gene expression of CXCL12 in WT and Caspase-3^−/− BA-MSCs by qRT-PCR (n = 6), and ELISA analysis in the supernatants after culture for 7 days (n = 4). C Gene expression of G-CSF, M-CSF, GM-CSF, and IL-6 in WT and Caspase-3^−/− BA-MSCs by qRT-PCR (n = 3–4). D Gene expression of NLRP3 in WT and Caspase-3^−/− BA-MSCs by qRT-PCR (n = 5). E Western blot analysis of the expression of Caspase-3, NLRP3, and Caspase-1 in WT and Caspase-3^−/− BA-MSCs (n = 3). All data are presented as mean ± SEM. ns: not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001