Fig. 6
From: Umbilical cord blood plasma-derived exosomes as a novel therapy to reverse liver fibrosis
UCB-Exo inhibit cell proliferation via suppressing TGF-β/ID1 signaling. LX2 cells were treated with 20 μg/mL of UCB-Exo or PB-Exo with/without 5 ng/mL of TGF-β for 24 h and cell proliferation was determined by CyQUANT assay kit (A). Western blotting analysis of phospho-Smad2/3, Smad2/3, and ID1 expressions in LX2 cells pretreated with 20 μg/mL of UCB-Exo or PB-Exo for 24 h, then stimulated by TGF-β for different durations (B). Quantification of western blot results was performed by calculating the ratio of pSmad2/3 to Smad2/3 (C) and the ratio of ID1 to GAPDH (D). Immunohistochemical staining of liver sections for ID1 (brown color) (E). Data are presented as the mean ± SEM. ***p < 0.001