Table 1 Primer sequences
From: Umbilical cord blood plasma-derived exosomes as a novel therapy to reverse liver fibrosis
Target gene | Primer sequence (5–3′) | Amplicon size (bp) |
|---|---|---|
MMP-13 | For: AACCCTAAGCACCCCAAAACA Rev: GGTCAAAAACAGTTCAGGCTCAA | 150 bp |
Pro-Collagen type I-α1 | For: CCAGCGGTGGTTATGACTTCA Rev: GCTGCGGATGTTCTCAATCTG | 167 bp |
MMP-2 | For: ACCATGCGGAAGCCAAGAT Rev: GCCCGAGCAAAAGCATCAT | 155 bp |
MMP-9 | For: CCTACTGCTGGTCCTTCTG Rev: GGCTTCCTCCGTGATTCG | 157 bp |
TIMP-1 | For: CATGGAAAGCCTCTGTGGAT Rev: CTCAGAGTACGCCAGGGAAC | 132 bp |
TIMP-2 | For: CACAGACTTCAGCGAATGGA Rev: CCAGCATGAGACCTCACAGA | 124 bp |
TGF-β | For: TTGCCCTCTACAACCAACACAA Rev: GGCTTGCGACCCACGTAGTA | 103 bp |