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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Nanog/NFATc1/Osterix signaling pathway-mediated promotion of bone formation at the tendon–bone interface after ACL reconstruction with De-BMSCs transplantation

Fig. 5

Mechanism underlying the enhanced osteogenic differentiation ability of De-BMSCs. a and b Effect of Si-NFATc1 in De-BMSCs. c Staining with alizarin red S and staining for alkaline phosphatase to detect the osteogenic differentiation of BMSCs and De-BMSCs. d mRNA expression of COL1A1. e Protein expression of COL1A1. f mRNA expression of OCN. g Protein expression of OCN. h mRNA expression of OPN. i Protein expression of OPN. j mRNA expression of Nanog. k Protein expression of Nanog. l mRNA expression of NFATc1. m Protein expression of NFATc1. n mRNA expression of Osterix. o Protein expression of Osterix. p Effects of Si-NFATc1 on the interaction of NFATc1 and Osterix. Data represent the mean ± SD (n = 3). *p < 0.05, **p < 0.01. BMSCs, bone marrow mesenchymal stem cells; De-BMSCs dedifferentiated osteogenic bone marrow mesenchymal stem cells, COL1A1 α1 chain of type I collagen, OCN osteocalcin, OPN osteopontin, NFATc1 nuclear factor of activated T-cells c1

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Stem Cell Research & Therapy

ISSN: 1757-6512