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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Targeted integration of EpCAM-specific CAR in human induced pluripotent stem cells and their differentiation into NK cells

Fig. 3

CAR expression after the differentiation of iPSCs into iNK cells. A RT-PCR analysis on the expression of pluripotent markers Oct4, Sox2 and Nanog in parental iPSCs (Lane 2), genetically modified iPSCs (Lane 3), and differentiated iNK cells (Lane 4). A 100-bp DNA ladder is included as a reference marker in Lane 1. B RT-PCR analysis showed that the expression of anti-EpCAM CAR was easily detectable after differentiation into iNK cells. C Flow cytometry analysis demonstrating anti-EpCAM CAR expression upon NK cell differentiation. The CAR expression was examined by staining with a biotinylated primary antibody against mouse IgG Fab fragment, followed by an APC-streptavidin conjugate. Unstained and APC-streptavidin conjugate-stained iPSCs and iNK cells are included as negative controls. D Flow cytometry analysis of GFP expression to demonstrate a diminished GFP expression in differentiated iNK cells

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Stem Cell Research & Therapy

ISSN: 1757-6512