Fig. 4
Angiogenic effect of sevoflurane preconditioned MSCs in vitro. Representative images (scale bar = 200 μm) and quantification of nodes and branches length (that indicated tube formation) of HUVECs co-cultured with sevoflurane preconditioned MSCs in Matrigel for 4 or 6 h (a). LDH, IL10, IL-1β and TNF-α levels of HUVECs co-cultured with sevoflurane preconditioned MSCs under H/R (b). H + O2, HUVECs under normoxia; HM + O2, HUVECs co-cultured with MSCs under normoxia; HMS + O2, HUVECs co-cultured with sevoflurane preconditioned MSCs under normoxia; H-O2, HUVECs under H/R; HM-O2, HUVECs co-cultured with MSCs under H/R; HMS-O2, HUVECs co-cultured with sevoflurane preconditioned MSCs under H/R. Data are shown as Mean ± SEM, n = 3 per group. *P < 0.05, **P < 0.01, ***P < 0.001 versus H + O2; &&P < 0.01 versus HM + O2; #P < 0.05, ##P < 0.01, ###P < 0.001 versus H-O2; +P < 0.05 versus HM-O2