Fig. 5

SPION detection and βIII-tubulin, GABA, DARPP32 immunostaining. A–C The presence of iron was detected by Prussian blue staining. A, B The appearance of iron in the hESC injected site (A) and away from the injected site (B). C In the contralateral ESCs group, the iron could also be observed in the ipsilateral site. Scale bar: 100 μm. D–F The transplanted stem cells were positive with βIII-tubulin (D), GABA (E), and DARPP32 (F). Scale bar: D–F 200 μm