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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Genome-wide differential expression profiling of lncRNAs and mRNAs in human induced pluripotent stem cell-derived endothelial cells exposed to e-cigarette extract

Fig. 6

Assessment of metabolic changes in iPSC-ECs following e-cig treatment. A qPCR validation of FAO genes following e-cig treatment (6.5 TPE). B FAO, C glycolysis, and D glucose uptakes of iPSC-ECs following e-cig exposure (6.5 TPE) for 24 h (n = 3). Representative images and corresponding quantitative data of control and e-cig-treated cells taken with a fluorescence microscope (E, F) or flow cytometry (G). Live cells were stained with MitoTracker Red and MitoView Green. H ATP levels in cells treated with vehicle or e-cig (6.5 TPE) and/or 1.5 µM of oligomycin A for 48 h. Data are represented as mean ± SD, and * and ** indicate p < 0.05 and p < 0.001, respectively. Scale bars = 100 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512