Fig. 2
From: PINK1 deficiency impairs osteoblast differentiation through aberrant mitochondrial homeostasis
PINK1 is required for osteoblast differentiation. a MC3T3-E1 cells were induced to differentiate, followed by staining of the cells using alkaline phosphate (Alp) and Alizarin Red S (ARS). b mRNA levels of Pink1 and other markers of mature osteoblasts in MC3T3-E1 cells after osteogenic induction. c Protein levels of PINK1 and other osteogenic markers were analyzed using western blotting after osteogenic induction. d Alp and ARS staining of MC3T3-E1 cells that were differentiated after treatment with Pink1 siRNA. Histograms show ALP activity and quantification of Alizarin red S staining by spectrophotometry. e The effects of Pink1 siRNA on the mRNA levels of marker genes of mature osteoblasts. f The effects of Pink1 siRNA on protein levels of osteogenic markers in cells. Bsp, bone sialoprotein; Ocn, osteocalcin; Opn, osteopontin; Pink1, PTEN-induced kinase 1